Danube Strategy involves creating synergies between different sectors (tourism, environmental protection, agriculture and forestry), promoting solutions and contributing to the prosperity of social, economic and cultural sustainable development. It requires multi- interdisciplinary problem solving biodiversity conservation and environmental protection. Agro- biodiversity conservation and means must be assessed according to international risk categories, taking into account the culture, traditions and local lifestyle. Fundamental aspects of the protocols will be to assess species and breeds endangered and their influence on specific ecosystems of the Danube and Danube Delta to promote local tradition and cultural heritage. Our studies will have to reveal new interdependencies between socio- economic phenomena that occur in the context of regional sustainable development and identify viable solutions to ensure the conservation of agro- biodiversity as part of the natural and cultural heritage of the Danube Delta . Need of cooperation between Danube countries and between local authorities and research entities to conserve natural and cultural heritage that can be achieved based on eco-bio-diplomacy. Need of cooperation between Danube countries and between local authorities and research entities to conserve natural and cultural heritage that can be achieved based on eco-bio-diplomacy.